Download e-book for kindle: In Situ Hybridization in Electron Microscopy (Methods in by Gerard Morel,Annie Cavalier,Lynda Williams
By Gerard Morel,Annie Cavalier,Lynda Williams
° hybridization on ultrathin sections of tissue embedded in hydrophilic resin (post-embedding method)
° hybridization sooner than embedding (pre-embedding)
° hybridization on ultrathin sections of frozen tissue (frozen tissue method).
For each one process, the various levels are defined intimately: the education of tissue, pretreatment, hybridization, and visualization of the hybridization items.
The e-book combines conception and perform, beginning with the elemental ideas, then breaking down the experimental procedure into successive steps illustrated through various diagrams, specific protocols, and tables. this can be all performed in a layout that makes use of parallel columns to exhibit priceless reviews subsequent to the speculation and sensible information along each one level of the protocol. also, the precis tables give you the standards for selecting the probe sort and procedure, and an in depth index aids within the look for details. in situ Hybridization In Electron Microscopy is a vital significant other for making use of those equipment on the electron microscopic level.